绵羊Otx-2基因的分子克隆、组织表达及其与季节性繁殖关系的研究

doi:10.11843/j.issn.0366-6964.2015.05.003

Abstract

Abstract:

In order to understand the effect of Otx-2 gene on sheep reproductive regulation，the part of CDS（Coding region） Otx-2，MT1，KISS-1，GnRH genes were cloned from Altay sheep hypothalamus tissue.Semi-quantitative RT-PCR was used to analyze the tissues（heart，liver，spleen，lung，kidney，large intestine，small inestine，stomach，brain，epencephalon，uterus，hypophysis，pineal body，hypothalamus，thyroid） expression profile of the genes.The expression patterns of Otx-2，MT1，KISS-1 and GnRH genes in hypothalamus with different periods were further investigated by real-time fluorescence quantitative PCR.The results showed that Otx-2 and KISS-1，MT1 mRNAs were expressed in many tissues tested，which hypothalamus showed higher expression levels for Otx-2 and KISS-1.In addition，the Otx-2 gene showed a similar expression pattern to KISS-1 and GnRH，which were significantly higher in the breeding seasons than that in the non-breeding seasons（P<0.01），and also significantly higher in the night than in the day time（P<0.01）.It was concluded that Otx-2 gene might take part in seasonal reproduction in sheep.

CLC Number:

S826.2

GUO Ruo-ting，LU Hui-wen，OU Ke-peng，WANG Ying，LIU Yi，GU Zhen-zhen，LIU Xiao-jun. Cloning and Tissues Expression Analysis of Otx-2 and the Associations with Seasonal Breeding in Sheep[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(5): 696-703.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2015.05.003

https://www.xmsyxb.com/EN/Y2015/V46/I5/696

References

［1］GREIVES T J，MASON A O，SCOTTI M，et al.Environmental control of kisspeptin：implications for seasonal reproduction［J］.Endocrinology，2007，148（3）：1158-1166.
［2］DE ROUX N，GENIN E，CAREL J C，et al.Hypogonadotropic hypogonadism due to loss of function of the KiSS1-derived peptide receptor GPR54［J］.Proc Natl Acad Sci USA，2003，100（19）：10972-10976.
［3］SEMINARA S B，MESSAGER S，CHATZIDAKI E E，et al.The GPR54 gene as a regulator of puberty［J］.N Engl J Med，2003，349（17）：1614-1627.
［4］PIELECKA-FORTUNA J，CHU Z，MOENTER S M.Kisspeptin acts directly and indirectly to increase gonadotropin-releasing hormone neuron activity and its effects are modulated by estradiol［J］.Endocrinology，2008，149：1979-1986.
［5］ROA J，VIGO E，CASTELLANO J M，et al.Opposite roles of estrogen receptor（ER）-alpha and ERbeta in the modulation of luteinizing hormone responses to kisspeptin in the female rat：implications for the generation of the preovulatory surge［J］.Endocrinology，2008，149：1627-1637.
［6］SHAHAB M，MASTRONARDI C，SEMINARA S B，et al.Increased hypothalamic GPR54 signaling：a potential mechanism for initiation of puberty in primates［J］.Proc Natl Acad Sci USA，2005，102：2129-2134.
［7］THOMPSON E L，PATTERSON M，MURPHY K G，et al.Central and peripheral administration of kisspeptin-10 stimulates the hypothalamic-pituitary-gonadal axis［J］.J Neuroendocrinol，2004，16：850-858.
［8］NOVAIRA H J，NG Y，WOLFE A，et al.Kisspeptin increases GnRH mRNA expression and secretion in GnRH secreting neuronal cell lines［J］.Mol Cell Endocrinol，2009，311：126-134.
［9］WEAVER D R，REPPERT S M.The Mel1a melatonin receptor gene is expressed in humans uprachiasmatic nuclei［J］.Neuroreport，1996，8（1）：109-112.
［10］ANSEL L，BOLBOREA M，BENTSEN A H，et al.Differential regulation of KISS1 expression by melatonin and gonadal hormones in male and female Syrian hamsters［J］.Biol Rhythms，2010，25（2）：81-91.
［11］薛海龙，宋兴超，杨镒峰，等.褪黑激素对动物繁殖活动的影响及调控机理［J］.特产研究，2014，1：64-66.
XUE H L，SONG X C，YANG Y F，et al.Effect of melatonin on animal reproduction and the mechanism of action［J］.Special Wild Economic Animal and Plant Research，2014，1：64-66.（in Chinese）
［12］NOVAIRA H J，FADOJU D，DIACZOK D，et al.Genetic mechanisms mediating kisspeptin regulation of GnRH gene expression［J］.J Neurosci，2012，32：17391-17400.
［13］SIMEONE A，ACAMPORA D，MALLAMACI A，et al.A vertebrate gene related to orthodenticle contains a homeodomain of the bicoid class and demarcates anterior neuroectoderm in the gastrulating mouse embryo［J］.EMBO J，1993，12：2735-2747．
［14］ARENDT D.Evolution of eyes and photoreceptor cell types［J］.Int J Dev Biol，2004，477-478.
［15］DATEKI S，FUKAMI M，SATO N，et al.Otx2 mutation in a patient with anophthalmia，short stature，and partial growth hormone deficiency：functional studies using the IRBP，HESX1，and POU1F1 promoters［J］.J Clin Endocrinol Metab，2008，93：3697-3702.
［16］DATEKI S，KOSAKA K，HASEGAWA K，et al.Heterozygous orthodenticle homeobox 2 mutations are associated with variable pituitary phenotype［J］.J Clin Endocrinol Metab，2010，95：756-764.
［17］GOTTSCH M，CUNNINGHAM M，SMITH J，et a1.A role for kisspeptins in the regulation of gonedotropin 8 eclwdon in the mouse［J］.Endocrinology，2004，145（9）：4073-4077.
［18］KAUFFMAN，GOTTSCH M，ROA J，et a1.Sexual differentiation of KISS-l gene expression in the brain of the rat［J］.Endocrinology，2007，148（4）：1774-1783.
［19］SMITH J，CANNINGHAM M，RISSMAN B，et al.Regulation of KISS-l gene expression in the brain of the female mouse［J］.Endocrinology，2005，146（9）：3686-3692.
［20］SMITH J，DUNGAN H，STOLL E，et a1.Differential regulation of KISS-l mRNA expression by sex steroids in the brain ofthe male mouse［J］.Endocrinology，2005，146（7）：2976-2984.
［21］KOTANI M，DETHEUX M，ANDENBOGAERDE A，et a1.The metastasis suppressor gene KISS-1 encodes kisspeptins，the natural ligands of the orphan G protein-coupled receptor GPR54［J］.J Biol Chem，2001，276（37）：34631-34636.
［22］MUI A，CHAMBERLAIN L，EISHOURBAGY N，et al.AXORl2，a novel human G protein-coupled receptor，activated by the peptide KISS-l［J］.J Biol Chem，2001，276（31）：28969-28975.
［23］CASTELLANO J，GAYTAN M，ROA J，et a1.Expression of KiSS-l in rat ovary：putative local regutator of ovulation?［J］.Endocrinology，2006，147（10）：4852-4862.
［24］MESSAGER S，CHATZIDAKI E E，MA D，et al.Kisspeptin directly stimulates gonadotropin-releasing hormone release via G protein-coupled receptor 54［J］.Proc Natl Acad Sci USA，2005，102（5）：1761-1766.
［25］TENA-SEMPERE M.GPR54 and kisspeptin in reproduction［J］.Hum Reprod Update，2006，12（5）：631-639.
［26］TOVAR S，VAZQUEZ M，NAVARRO V，et al.Effects of single or repeated intravenous administration of kisspeptin upon dynamic LH secretion in conscious male rats［J］.Endocrinology，2006，147（6）：2696-2704.
［27］KADOKAWA H，MATSUI M，HAYASHI K，et al.Peripheral administration of kisspeptin-10 increases plasma concentrations of GH as well as LH in prepubertal Holstein heifers［J］.J Endocrinol，2008，196（2）：331-334.
［28］HAN S，GOTTSCH M，LEE K，et al.Activation of gonadotropin-releasing hormone neurons by kisspeptin as a neuroendocrine switch for the onset of puberty［J］.J Neurosci，2005，25（49）：11349-11356.
［29］CASTELLANO J，NAVARRO V，FERNANDEZ-FERNANDEZ R，et al.Ontogeny and mechanisms of action for the stimulatory effect of kisspeptin on gonadotropin-releasing hormone system of the rat［J］.Mol Cell Endocrinol，2006，26：257-258.
［30］李隐侠，张俊，钱勇，等.KISS-1基因多态性与苏淮山羊产羔数的关联分析［J］.畜牧兽医学报，2014，45（12）：1917-1923.
LI Y X，ZHANG J，QIAN Y，et al. KISS-1 gene polymorphisms and its association with litter sizes in Suhuai goats［J］.Acta Veterinaria et Zootechnica Sinica，2014，45（12）：1917-1923.（in Chinese）
［31］王军，孙蕾，吕文发，等.KISS-1/GPR54系统在不同发情周期母羊下丘脑中的表达规律［J］.西北农林科技大学学报（自然科学版），2011，12：49-52，59.
WANG J，SUN L，LÜ W F，et al.Expression of KISS-1/GPR54 system in the ewe hypothalamus during the estrous cycle［J］.Journal of Northwest A & F University（Nature Science Edition）， 2011，12：49-52，59.（in Chinese）
［32］BLAZER I，BEHRMANN G，HARDELAND R.Evolutionary significance of the presence of melatonin in unicells and plants［J］.Ibid，1995，14：2-3.
［33］葛仕豪.济宁青山羊生殖激素分泌规律及GnRHR在垂体和卵巢的免疫组化研究［D］.泰安：山东农业大学，2007.
GE S H.Study on the endocrine mechanism of reproduction hormone of the immunohistochemistry of GnRHR in pituitary and oavry of Jining Gary goat［D］.Taian：Shandong Agricultural University，2007.（in Chinese）
［34］马友记，李发弟，张利.褪黑素对动物生殖调控机理的研究进展［J］.中国草食动物，2007，Sl：85-88.
MA Y J，LI F D，ZHANG L.Melatonin on animal reproductive regulation mechanism research［J］.China Herbivores，2007，Sl：85-88.（in Chinese）
［35］杜玉珍，童建.视交叉上核中褪黑素受体mRNA的昼夜节律性表达［J］.苏州大学学报（医学版），2002，22（1）：1-3.
DU Y Z，TONG J.Circadian expression of melatonin receptor mRNA in suprtchiasmtic nucleus［J］.Suzhou University Journal of Medical Science，2002，22（1）：1-3.（in Chinese）
［36］MARTINE M，AGNES D，BENOIT M.MTNR1A melatonin receptors in the ovine premammillary hypothalamus：day-night variation in the expression of the transcripts［J］.Biol Reprod，2005，72（2）：393-398.
［37］DIACZOK D，ROMERO C，ZUNICH J，et al.A novel dominant negative mutation of Otx2 associated with combined pituitary hormone deficiency［J］.J Clin Endocrinol Metab，2008，93（11）：4351-4359..
［38］TAJIMA T，OHTAKE A，HOSHINO M，et al.Otx2 loss of function mutation causes anophthalmia and combined pituitary hormone deficiency with a small anterior and ectopic posterior pituitary［J］.J Clin Endocrinol Metab，2009，94：314-319.
［39］邓锦波，徐晓波，范文娟.小脑发育及其基因调节［J］.解剖科学进展，2010，16（2）：181-186.
DENG J B，XU X B，FAN W J.Development and gene regulation of cerebellum［J］.Progress of Anatomical Sciences，2010，16（2）：181-186.（in Chinese）
［40］ROA J，VIGO E，CASTELLANO J，et al.Hypothalamic expression of KISS-1 system and gonadotropin-releasing effects of kisspeptin in different reproductive states of the female rat［J］.Endocrinology，2006，147（6）：2864-2878.

[1]	WANG Haibo, ZHAN Jinshun, GU Zhiyong, CHEN Xinfeng, PAN Yue, JIA Haobin, ZHONG Xiaojun, LI Kairong, ZHAO Shengguo, HUO Junhong. Comparative Study on Meat Quality Characteristics of Three-Way Hybrid Sheep Charolais×Duper×Hu and Charolais×Australian White×Hu and Hu Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 110-119.
[2]	HAN Haoyuan, LI Shikai, YANG Ruiqiao, LI Manman, LI Jun, HA Si, ZHAO Jinyan, WEI Hongfang, QUAN Kai. Mining Key Candidate Genes for High Reproduction Performance of Huai Goats Based on Transcriptome Sequencing [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 5077-5090.
[3]	LI Dengpan, MA Keyan, HAN Jintao, BAI Yaqin, LI Taotao, MA Youji. Detection and Analysis of Whole Genome Selection Signal of Yongdeng Qishan Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4577-4588.
[4]	ZHAO Xueyang, LI Danni, WANG Yuchen, GUO Lei, WANG Li, HUANG Jie, JIAO Yiqiang, AN Xiaopeng, ZHANG Xiyun, ZHANG Lei, SONG Yuxuan. GWAS Analysis of Lambing Traits in East Friesian and Hu Crossbred Sheep and Verification of Candidate Gene GRID2 [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4625-4635.
[5]	ZHANG Hanyue, ZHAO Dan, LIANG Yu, ZHAO Bishi, FAN Mengdan, QIAO Liying, LIU Jianhua, YANG Kaijie, PAN Yangyang, LIU Wenzhong. miR-150 Regulates Ovine Preadipocyte Differentiation by Targeting AOC3 [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3262-3274.
[6]	LIU Linli, PENG Xuelan, LI Bo, CHENG Lefan, CIREN Lamu, ZHANG Enping. Effect of Overexpression of UCP3 Gene on the Differentiation of Sahu Sheep Preadipocytes [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3275-3285.
[7]	WANG Wannian, CHEN Sijia, GAO Jinrong, WEN Zhonghao, YUAN Mengjiao, ZHANG Hongzhi, PANG Zhixu, QIAO Liying, LIU Wenzhong. Simulation Study on Genomic Selection of Sex-limited Traits Using Multilayer Perceptron in Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2824-2835.
[8]	DONG Yajie, HAO Xiaojing, WU Jinqiang, WANG Rong, ZHANG Pengxiang, WANG Haidong, HE Xiaoyan. Exploration of the Effect of SHH on Wool Bending through Krox20 Regulation of IGFBP5 Expression Based on Sheep Keratinocytes [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2365-2375.
[9]	BI Yazhen, SHANG Mingyu, HU Wenping, ZHANG Li. Correlation and Regression Analysis among Growth Traits and Association Analysis of TRHDE Gene Polymorphism with Growth Traits in Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1415-1428.
[10]	LI Yinxia, YA Shengjiang·Nasier, SAI Like·Duman, QIAN Yong, CAO Shaoxian, WANG Weilie, MENG Chunhua, ZHANG Jun, ZHANG Jianli. Evaluation of Genetic Diversity and Genetic Structure in Kirgiz Sheep Population Based on SNPs Chip [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(2): 572-583.
[11]	LI Ling, LI Yefang, LIANG Benmeng, SUN Yujiang, MA Yuehui, MA Qing, JIANG Lin, LIU Shuqin. Paternity Identification of Tan Sheep Based on SNP Markers [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(9): 2912-2919.
[12]	ZHOU Shiwei, WU Tingjie, WANG Qian, CAI Bei, HAN Saizheng, WU Yanhu, WANG Xiaolong, CHEN Yulin. Establishment of the Efficient Method to Detect the FecB Mutation and the Construction of the Prolific Tan Sheep Population [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(9): 2920-2929.
[13]	DING Ziqiang, GE Wenbo, DUAN Hongwei, LÜ Jianshu, ZENG Jianlin, WANG Wenjuan, NIU Tian, ZHANG Yong, ZHAO Xingxu, HU Junjie. Dihydrotestosterone Affects Sheep Uterine Function through Regulating Progesterone, Oestrogen and Apoptosis [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(9): 2993-3005.
[14]	LI Tingting, LIU Qiuyue, LI Xiangchen, WANG Haitao. Research Progress and Applications of Genes Associated with Economic Traits in Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(8): 2417-2434.
[15]	XU Songwei, XIANG Hua, ZHANG Huanrong, YANG Lu, WANG Xianjun, SALANG Wenzhu, ZHU Jiangjiang. Effects of Interfering SERBP1 on the Cell Proliferation, Cell Cycle and Apoptosis in Goat Turbinate Bone Primary Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(8): 2708-2720.

Cloning and Tissues Expression Analysis of Otx-2 and the Associations with Seasonal Breeding in Sheep

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments